Welcome Guest
Sign In
Register
Products
Popular Categories
Antibody Search
ELISA Kit Search
Biomolecules
Flow Cytometers
Thermal Cyclers
Microscopes
Transfection Reagents
Featured Products
New Products
Articles
Resources
eBooks
Bench Tips
Tech Insights
Editorial Articles
News
Product Reviews
Future Lab
Multimedia
Biocompare Webinars
Immunotherapy
Reproducibility
Transformative Technologies
Podcasts
Tech Talks
Bench Tip Videos
All Videos and Webinars
Life Science Webinars
Write a Review
Antibodies
Kits/Reagents
Equipment
Services
Resources
Promotions
Events
AACR SPOTLIGHT
Register
Sign In
Biocompare
Products
Cloning and Expression
Enzymes
Lys-C, Mass Spec Grade
Supplier Images
Lys-C, Mass Spec Grade
Lys-C, Mass Spec Grade
Lys-C, Mass Spec Grade from Promega
Be the first to write a review!
Citations:
(26)
Supplier Page
Supplier Page from
Promega for
Lys-C, Mass Spec Grade
Get Pricing
Ask a Question
Description
Lys-C Mass Spec Grade digests specifically at the carboxyl side of lysine and has optimal activity at pH 7-9. Lys-N Mass Spec Grade cleaves at the amino side of lysine and retains proteolytic activity under strong denaturing conditions
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Lys-C, Mass Spec Grade
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
VA1170
Quantity
20microg
Type
Enzyme
Add to Compare List
Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (26)
(1)
Multi-modal proteomic characterization of lysosomal function and proteostasis in progranulin-deficient neurons
Molecular Neurodegeneration
November 16, 2023
Saadia Hasan, Michael S. Fernandopulle, Stewart W. Humble, Ashley M. Frankenfield, Haorong Li, Ryan Prestil, Kory R. Johnson, Brent J. Ryan, Richard Wade-Martins, Michael E. Ward,
et al.
(IAA) for 30 min in a ThermoMixer shaking at 800 g at 37 °C. Proteins were digested with LysC (
Promega
#
VA1170
) at
1:30
(enzyme: protein) ratio for 16 h at 37 °C and quenched with 10% trifluoroacetic acid (TFA)
(2)
Analysis of the N-glycosylation profiles of the spike proteins from the Alpha, Beta, Gamma, and Delta variants of SARS-CoV-2
Analytical and Bioanalytical Chemistry
August 1, 2023
Dongxia Wang, Jakub Baudys, Sarah H. Osman, John R. Barr
trypsin (Cat#XV528X,
Mass Spectrometry
Grade), chymotrypsin (Cat#
V1061
,
Sequencing
Grade), Lys-C (Cat#
VA1170
,
Mass Spectrometry
Grade), and
Asp
-N (Cat#V162A,
Sequencing
Grade), were obtained from
Promega
(Madison,
(3)
Flt1 produced by lung endothelial cells impairs ATII cell transdifferentiation and repair in pulmonary fibrosis
Cell Death & Disease
July 15, 2023
Maria Concetta Volpe, Giulio Ciucci, Giulia Zandomenego, Roman Vuerich, Nadja Anneliese Ruth Ring, Simone Vodret, Francesco Salton, Pietro Marchesan, Luca Braga, Thomas Marcuzzo,
et al.
as described [18].
Mass spectrometry
Protein samples (20 μg) were digested with Lys-C (
Promega
, #
VA1170
), desalted and lyophilized for
LC-MS/MS
analysis on an
Easy-nLC 1200 system
(
Thermo Fisher Scientific
). Statistical
(4)
Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases to dissect their protein substrates and glycosylation sites in cells
STAR Protocols
March 1, 2023
Beatriz Calle, Edgar Gonzalez-Rodriguez, Keira E. Mahoney, Anna Cioce, Ganka Bineva-Todd, Omur Y. Tastan, Chloe Roustan, Helen Flynn, Stacy A. Malaker, Benjamin Schumann
,
Thermo Fisher Scientific
,Thermo Fisher Scientific Cat# 15338100 "
Lys-C, Mass Spec Grade
","Promega (Madison, USA)",
Promega
Cat#
VA1170
Ni-NTA® Agarose beads,"Qiagen (Hilden, Germany)",
Qiagen
Cat#
30210
Nuclease free water,Thermo Fisher
(5)
Multi-modal Proteomic Characterization of Lysosomal Function and Proteostasis in Progranulin-Deficient Neurons
bioRxiv : the preprint server for biology
February 24, 2023
Hasan S, Fernandopulle MS, Humble SW, Frankenfield AM, Li H, Prestil R, Johnson KR, Ryan BJ, Wade-Martins R, Ward ME,
et al
.
(IAA) for 30 min in a ThermoMixer shaking at 800 g at 37°C. Proteins were digested with LysC (
Promega
#
VA1170
) at
1:30
(enzyme:protein) ratio for 16 hours at 37°C and quenched with 10% trifluoroacetic acid (TFA)
(6)
Protein Expression of Amino Acid Transporters Is Altered in Isolated Cerebral Microvessels of 5xFAD Mouse Model of Alzheimer’s Disease
Molecular Neurobiology
February 1, 2023
Elena Puris, Liudmila Saveleva, Izaque de Sousa Maciel, Katja M. Kanninen, Seppo Auriola, Gert Fricker
Tosylphenylalanylchloromethyl ketone-treated trypsin (
VA9000
),
Protease
-Max surfactant (
V2072
), and lysyl endopeptidase (Lys-C,
VA1170
) were purchased from
Promega
(Madison, WI, USA). A mixture of standards (product number MSK-A2-1.2)
(7)
Increasing the throughput of sensitive proteomics by plexDIA
Nature Biotechnology
January 1, 2023
Jason Derks, Andrew Leduc, Georg Wallmann, R. Gray Huffman, Matthew Willetts, Saad Khan, Harrison Specht, Markus Ralser, Vadim Demichev, Nikolai Slavov
0.3 U μl−1 of Trypsin Gold (
Promega
,
V5280
) to
1:25
ratio of substrate:protease and LysC (
Promega
,
VA1170
) to
1:40
ratio of substrate:protease and then incubated at 37 °C for 18 hours.
E. coli
and
S. cerevisiae
(8)
Co-display of diverse spike proteins on nanoparticles broadens sarbecovirus neutralizing antibody responses
iScience
December 22, 2022
Mitch Brinkkemper, Tim S. Veth, Philip J.M. Brouwer, Hannah Turner, Meliawati Poniman, Judith A. Burger, Joey H. Bouhuijs, Wouter Olijhoek, Ilja Bontjer, Jonne L. Snitselaar,
et al.
L4509
Tris
(2-carboxyethyl)phosphine,Sigma-Aldrich,Cat# SBR00051 Chloroacetamide,Sigma-Aldrich,Cat# C0267 Trypsin,
Promega
,Cat#
V5117
LysC,
Promega
,Cat#
VA1170
SARS-CoV
peptide 1 DGIYFAATEK,Pepscan,N/A
SARS-CoV
peptide 2 AISQIQESLTTTSTALGK,Pepscan,N/A
SARS-CoV-2
(9)
Increased Expression and Activity of Brain Cortical cPLA2 Due to Chronic Lipopolysaccharide Administration in Mouse Model of Familial Alzheimer’s Disease
Pharmaceutics
November 10, 2022
Mikko Gynther, Mariana Leal Estrada, Sanna Loppi, Paula Korhonen, Katja M. Kanninen, Tarja Malm, Jari Koistinaho, Seppo Auriola, Gert Fricker, Elena Puris
ketone-treated trypsin (#
VA9000
), Protease Max surfactant (#
V2072
), and lysyl endopeptidase (Lys-C, #
VA1170
) were purchased from
Promega
(Madison, WI, USA). BioRad DC
Protein Assay
was purchased from EnVision
(10)
Targeting pancreatic cancer by TAK-981: a SUMOylation inhibitor that activates the immune system and blocks cancer cell cycle progression in a preclinical model
Gut
November 1, 2022
Sumit Kumar, Mark J A Schoonderwoerd, Jessie S Kroonen, Ilona J de Graaf, Marjolein Sluijter, Dina Ruano, Román González-Prieto, Matty Verlaan-de Vries, Jasper Rip, Ramon Arens,
et al.
samples were alkylated using 5 mM chloroacetamide. Proteins were first digested with Lys-C (
Promega
,
VA1170
) in a
1:100
enzyme-to-protein ratio for 5 hours. Peptides were diluted with 50 mM ammonium bicarbonate
(11)
Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells
iScience
May 20, 2022
Iwasaki M, Kawahara Y, Okubo C, Yamakawa T, Nakamura M, Tabata T, Nishi Y, Narita M, Ohta A, Saito H,
et al
.
sarcosinate (SLS),WAKO,Cat# 192-10382 Sucrose,WAKO,Cat# 195-07925 "
Lys-C, Mass Spec Grade
",
Promega
,Cat#
VA1170
Sequencing Grade Modified Trypsin
(Lyophilized),
Promega
,Cat#
V5111
Ethyl acetate,WAKO,Cat# 051-00356 Acetonitrile,WAKO,Cat#
(12)
Mitochondrial calcium uniporter deletion prevents painful diabetic neuropathy by restoring mitochondrial morphology and dynamics
Pain
March 1, 2022
Dale S. George et al.
iodoacetamide. Proteins were then digested for 3 hours with 2 μg of endoproteinase LysC (
Promega
Cat#
VA1170
) and subsequently digested overnight with 2 μg of trypsin (
Promega
V5280
). The digest was then acidified
(13)
3-hydroxy-L-kynurenamine is an immunomodulatory biogenic amine
Nature communications
July 21, 2021
Clement CC, D'Alessandro A, Thangaswamy S, Chalmers S, Furtado R, Spada S, Mondanelli G, Ianni F, Gehrke S, Gargaro M,
et al
.
Proteinase
inhibitor
cocktail (cat# 04693116001) was purchased from Roche. Trypsin (cat#
V5111
), Lys-C (cat#
VA1170
), and GluC (cat#
V1651
), all
sequencing
grade purity were purchased from
Promega
(Madison, WI, USA). Synthesis
(14)
PHAROH lncRNA regulates Myc translation in hepatocellular carcinoma via sequestering TIAR
eLife
May 18, 2021
Allen T Yu, Carmen Berasain, Sonam Bhatia, Keith Rivera, Bodu Liu, Frank Rigo, Darryl J Pappin, David L Spector
and 10 mM iodoacetamide for 30 min at 55°C, then digested overnight at 37°C with 1 μg Lys-C (
Promega
,
VA1170
) and dried in vacuo. Peptides were then reconstituted in 50 μl of 0.5 M TEAB/70% ethanol and labeled
(15)
Pleiotropic consequences of metabolic stress for the major histocompatibility complex class II molecule antigen processing and presentation machinery
Immunity
April 13, 2021
Clement CC, Nanaware PP, Yamazaki T, Negroni MP, Ramesh K, Morozova K, Thangaswamy S, Graves A, Kim HJ, Li TW,
et al
.
11088858001
XCR1
-Brilliant
Violet
510,
Biolegend
,Catalog #
148218
Endoproteinase Lys-C,
Promega
,Cat.#
VA1170
Trypsin,Promega,Catalog #
V5111
Glu-C,
Promega
,Catalog #
V1651
Acetonitrile Optima™
LC/MS
,Fisher Scientific,Catalog
(16)
PADI2‐Catalyzed MEK1 Citrullination Activates ERK1/2 and Promotes IGF2BP1‐Mediated SOX2 mRNA Stability in Endometrial Cancer
Advanced Science
March 1, 2021
Teng Xue et al.
re‐acting with 15 mm iodoacetamide for 45 min in the dark. The sample was then digested with Lys‐C (
VA1170
,
Promega
) at 37 °C for 16 h. Peptide samples were then desalted and lyophilized. The sample were then
(17)
One-shot analysis of translated mammalian lncRNAs with AHARIBO
eLife
February 17, 2021
Luca Minati et al.
temperature. Subsequently, proteins were digested at room temperature with 0.5 μg Lys-C (
Promega
, #
VA1170
) for 4 hr, after which the solution was diluted four times in 50 mM ammonium bicarbonate. Then, 1 μg
(18)
Coordinate Regulation of Ribosome and tRNA Biogenesis Controls Hypoxic Injury and Translation
Current Biology
January 1, 2021
Omar A. Itani et al.
Peptides, and Recombinant Proteins","Chemicals, Peptides, and Recombinant Proteins" Lys-C,
Promega
,Cat#:
VA1170
C18 Sep-Pak
solid phase extraction
cartridges,Waters,Cat#: WAT036945
Endoproteinase LysC
,
New England Biolabs
(19)
MaTAR25 lncRNA regulates the Tensin1 gene to impact breast cancer progression
Nature communications
December 22, 2020
Chang KC, Diermeier SD, Yu AT, Brine LD, Russo S, Bhatia S, Alsudani H, Kostroff K, Bhuiya T, Brogi E,
et al
.
10 mM iodoacetamide for 30 min at 55 °C, then digested overnight at 37 °C with 1 μg Lys-C (
Promega
,
VA1170
) and dried in vacuo. Peptides were then reconstituted in 50 μl of 0.5 M TEAB/70% ethanol and labeled
(20)
Noise Exposures Causing Hearing Loss Generate Proteotoxic Stress and Activate the Proteostasis Network
Cell reports
November 24, 2020
Jongkamonwiwat N, Ramirez MA, Edassery S, Wong ACY, Yu J, Abbott T, Pak K, Ryan AF, Savas JN.
87793
ProteasMax,
Promega
,Cat#
V2071
Sequencing grade modified Trypsin
,
Promega
,Cat#
V5111
Lys-C,
Promega
,Cat#
VA1170
Pierce
Trypsin
Protease,
Pierce
,Cat#
90058
Casein Blocking Buffer
,
Sigma
,Cat# B6429 "SpikeTides TQL
Page
<<
<
1
2
>
>>
Reviews
Be the First to Write a Review!
Lys-C, Mass Spec Grade from Promega
Supplier Page
View Company Product Page
Product Specs
Company Info
Citations
Reviews
Back To Top